To evaluate effect of biofertilizers on the yield and nutrients of Dracocephalum moldavica seeds, a field experiment was conducted based on randomized complete block design with four replications at the Agricultural and Natural Resources Research Center of West Azerbaijan Urmia-Iran in 2010. Treatments were biofertilizers (seed inoculation with nitrogen fixing bacteria (N­_b), phosphate solubilizing bacteria (P_b), sulfur oxidizing bacteria (S_b), N­_b+P_b, N_b+S_b, P_b+S_b, N­_b+P_b+S_b), chemical fertilizer (chemical origin of nitrogen+phosphorus+potassium (NPK)) and control (no fertilizer). Results indicated that the highest yield of seed (3366 kg ha^-1) belonged to chemical origin of NPK. Maximum seed nitrogen (3.74%) and manganese (0.0062 mg kg^-1) contents were obtained from N_b treatment. The highest percentage of sulfur (0.56%) and zinc (0.184 mg kg^-1) were obtained from S_b treatment. While the maximum iron content (0.62 mg kg^-1) was observed at N­_b+P_b+S_b. In conclusion seed inoculation with Thiobacillus (S_b) improves seed nutrients availability in calcareous soils.