Metacercariae of two new trematode species of the genus Austrodiplostomum are described from fishes of Tana Lake in Ethiopia: Austrodiplostomum sp. 1 from cranial cavity of Oreochromis niloticus and Austrodiplostomum sp. 2 from vitreous humor of Varicorhinus beso. New species metacercariae differs from A. mordax metacercariae in the shape of body, the size of body and organs, the shape of anterior extremity, and site of infection. This is the first record of Austrodiplostomum metacercariae from fish in Africa.