Today bioconversion is a promising method of processing plant waste into valuable products for rural households. The aim of this study was to assess the possibility of using a mixed substrate based on poplar leaves and buds after extraction of extractive substances for biological preparation. The study of the chemical composition of the substrate showed that 65.5% of it is limnogeology complex, which can serve as a source of carbon nutrition of microorganisms. Extractive substances are supplied by both water-and alcohol-soluble components, and the share of substances extracted by water comes to 77% of the amount. Also substrate can serve as a source of various trace elements. The mineral content is 8,6%.

The article presents the results of a study of the impact of the enzyme complex strains M99-9 and K6-15 micro-peach fungi of the genus Trichoderma on a mixed substrate. It is established that in the process of biodegradation with strains K6-15 M99-9 in the substrate reduced content of extractives 2.1-2.4 times that of the polysaccharide is 1.5-1.8 times, and ligninase substances 1.2-1.4 times. This was an accumulation of up to 9-12 % of humic substances. The decrease in the mass of the substrate during the cultivation of the strain M99-9 was 17.3 %, and the strain K6 – 15-30% less. The most productive on this substrate is strain K6-15. The growth of conidia within two weeks of cultivation for strain K6-15 was 16.6•10⁸ CFU/g and strain M99-9 – 11,5•10⁸ CFU/g. High yield of spores and formation of humic substances during cultivation of fungi of the genus Trichoderma makes it possible to use the vegetative part of a poplar as raw materials for receiving a biological product "Trichodermin" or humification of the soil.