The present investigation aimed to understand the role of ethylene diamine as a potential linker to observe distance dependent metal-enhanced bioluminescence (MEB) when placed in vicinity to gold nanoparticles (AuNPs). Four different types of AuNPs (AuNP1, AuNP2, AuNP3, AuNP4) were synthesized with maximum absorbance values of 0.99, 1.24, 1.21 and 1.38 respectively while the corresponding LSPR peaks (l_max) were 520 nm, 535 nm, 525 nm, and 525 nm. Luminescence enhancement up to 1.44 fold was observed when ethylene diamine was used as a linker in presence of EDC, ATP and AuNP1 while the sample consisting FMN, EDC and AuNP1 led to 1.3 fold of luminescence enhancement. It was noted that the AuNPs synthesized using silver nitrate as an additional component did not enhance luminescence in all the investigations. This approach can be substantiated to study more parameters which can positively influence MEB.