Paper deals with discussion of possibilities and directions of decreasing gaps between academic research and business. Barriers’ reduction and motivations’ improving within partnership cooperation between regional innovations ecosystems’ actors are taken into account.  Many researchers from developed and developing countries pay attention to the problems of interaction between universities and companies in order to find ways to increase effectiveness and academic research and make it results relevant to real demand. Development of partnership relations is known to be a factor which accelerates innovations’ creation and dissemination. Improvement of such relationship helps to increase possibilities of academic researchers, to adjust educational programs, to develop regional economy. Basing on international experience review paper determines main tendencies and perspectives of research – business cooperation as well as main directions of partnership cooperation support. Empirical part of the paper deals with the results of original pilot survey which uses data from a number of research, educational organizations and high tech companies form Novosibirsk region. The aim of the survey was to analyze success factors and main obstacles for the sustainable research business cooperation.  Qualitative methods including case studies, interviews, and surveys were used within research. It was found that despite the fact that all representatives of the considered sectors accept the necessity to develop partnership cooperation motivation differences and aims conflicts are still significant for the cooperation between different actors and even for relationships inside each organization. Besides well known barriers (lack of financial and other resources, personnel etc.) some other factors turned to be important. Low level of confidence and negative cooperation experience impede cooperation development. Personal issues and previous successful experience help to develop and improve partnerships. Such cases could be found. State support especially at regional level is quiet necessary; it is not limited to financial instruments. Support of communications which helps to increase level of confidence and to decrease “attrition costs” of cooperation has also great importance.